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1:5,000 dilution) were used to detect GAPDH, which functioned as an internal control for normalization

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Figure 1 4.1 Activation of Toll-like receptors by dysbiotic microbiome affects esophageal barrier structure, epithelial repair, and triggers inflammatory responses involved in GERD G- bacteria are key producers of lipopolysaccharide (LPS), which abnormally activates a variety of Toll-like receptors (TLRs) leading to epithelial barrier dysfunction and inflammatory responses leading to GERD